Incorporation of Glycine max Merrill Extract into Layered Double Hydroxide through Ion-Exchange and Reconstruction

Incorporation of Glycine max Merrill Extract into Layered Double Hydroxide through Ion-Exchange and Reconstruction

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We incorporated extract of Glycine max Merrill (GM), which is generally known as soybean, into a layered double hydroxide (LDH) nanostructure through two different methods, ion-exchange and reconstruction.Through X-ray diffraction, field-emission scanning electron microscopy, and zeta-potential measurement, GM moiety seemed to be simply attached on the surface of LDH read more by ion-exchange process, while the extract could be incorporated in the inter-particle pore of LDHs by reconstruction reaction.The quantification exhibited that both incorporation method showed comparable extract loading capacity of 15.6 wt/wt% for GM-LDH hybrid prepared by ion-exchange (GML-I) and 18.

6 wt/wt% for GM-LDH hybrid by reconstruction (GML-R).On the other hand, bioactive substance in both GM-LDH here hybrids, revealed that GML-R has higher daidzein content (0.0286 wt/wt%) compared with GML-I (0.0108 wt/wt%).

According to time-dependent daidzein release, we confirmed that GML-R showed pH dependent daidzein release; a higher amount of daidzein was released in pH 4.5 physiological condition than in pH 7.4, suggesting the drug delivery potential of GML-R.Furthermore, alkaline phosphatase activity and collagen fiber formation on human osteoblast-like MG-63 cells displayed that GML-R had superior possibility of osteoblast differentiation than GML-I.

From these results, we concluded that reconstruction method was more effective for extract incorporation than ion-exchange reaction, due to its pH dependent release property and alkaline phosphatase activity.